MINI-4-GAS automatic gas mixing module



MINI-4-GAS

LAMBDA gas-mixing module allows a flexible thermal massflow controlled supply of different gases (air, O2, CO2, N2, other gases) with individual gas flow paths for mammalian cell culture as well as high cell density microbial applications in the laboratory.

Customizable gas station with massflow gas flow controllers are specially designed to use with MINIFOR laboratory bioreactors and fermentors. E.g.: it allows control of the pH of a cell culture by the controlled addition of gaseous CO2, the control of dissolved oxygen by regulation of the airflow or any other gas with a suitable controller. However, it can also be used independently, since all its functions can be controlled from the front panel of the MASSFLOW.

MINI-4-GAS is a high quality and cost efficient customizable setup. Free selection of gas types: Air, Oxygen, Carbon dioxide, Nitrogen for high precision; (optional: Methane, Carbon monoxide, etc.)

Individual flow rates per gas:

MASSFLOW 5000: 0 – 5 l/min
MASSFLOW 500: 0 – 500 ml/min


The flow rate is regulated by a special proprietary proportional needle valve controlled by a microprocessor. The flow rate can be programmed and the transferred gas volume totalized with the optional FLOW INTEGRATOR.

Software control

The SIAM is automation PC software for laboratories in biotechnology and chemistry.

SIAM allows remote control and data storage during cell growth, production phase of fermentation, bio-trasformation and other processes in bioreactors and fermentors.

The extension of industrial fermentation software SIAM, MINI-4-GAS software allows a complete automatic gas-mix control for MINIFOR fermentor – bioreactor system and extension of PNet software for stand-alone MINI-4-GAS module.

MINIFOR bioreactor-fermentor dissolves the gases by micro-sparger, surface-aeration and an excellent stirring with “FISH-TAIL” discs, the most efficient gas-uptake without hydrodynamic shear stress to cells.

PID controller for each gas: Easy set-point adjustment; Low & High flow rate limits for each gas

Real-time trends: Visualization of the entire process

Data acquisition: Data storage for comparison of experiments, process optimization, calculations & gas transfer rates

No additional software license for adding further units and lab instruments

E.g.: 4-gas mix configuration with Air, O2, CO2, N2
Individual PID controllers change the CO2, Air and O2 flow rates depending on the actual and set point values
N2 to maintain a fixed total flow rate (N2 flow rate = set total flow rate - CO2 flow rate - Air flow rate - O2 flow rate)

Applications of MINI-4-GAS

MINI-4-GAS automatic gas mixing module can be used with MINIFOR fermentor and bioreactor and also as a stand-alone instrument.

Compared to most bio-transformations with quick growing yeast or bacteria, mammalian cell cultures due to their low specific growth rates have to be cultured for weeks or even months to achieve satisfying product yields.

MINIFOR bioreactor offers the “unique easy sterility” concept and excellent peristaltic pumps specially constructed for the long-term cell cultures. With the 4-gas-mixing-module, it maintains the optimal physiologic conditions of mammalian and stem cell cultures using the modern control strategies.

Controlling the critical process parameters will help to optimize the growth rates as well as to achieve the higher titers during the protein processing and hormone production.


2-GAS MIX

Optimal for pO2 control and O2 enrichment in high cell density microbial fermentation process and biofuel development



3-GAS MIX

Optimal for anaerobic fermentation



4-GAS MIX

Optimal for cell cultures, stem cells and tissue cultures

E.g.: Gas-mix for mammalian cell culture

pO2 control with air and O2 (O2 enrichment)

pH control by CO2

Constant total flow